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apc cy7 rat anti mouse cd11b  (Miltenyi Biotec)


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    Miltenyi Biotec apc cy7 rat anti mouse cd11b
    Flow cytometric analysis of microglia surface markers in SLE-serum, healthy-serum and ACSF-treated mice (n = 16, 14 and 10). a Cells were defined by gate R1 to eliminate unwanted events, such as cell debris. b Microglias were identified by their CD45 low <t>CD11b</t> + phenotype (R2). c Isotype for MHCII was used to define the gating of M1 cells. In this population, SLE-serum led to a significantly increased percentage of MHC-II + CD45 low CD11b + cells. d Isotype for CD206 was used to define the gating of M2 cells. In contrast, percentage of CD206 + was not significantly changed by SLE-serum treatment in the CD45 low CD11b + cells. Bars represent the mean ± SEM of mice groups received injection of SLE-serum, healthy-serum or ACSF. Pictures of c and d show representative histogram and flow dot plots of MHC-II and CD206 expression, respectively, in the CD45 low CD11b + population. *p < 0.05; **p < 0.01
    Apc Cy7 Rat Anti Mouse Cd11b, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apc cy7 rat anti mouse cd11b/product/Miltenyi Biotec
    Average 99 stars, based on 17 article reviews
    apc cy7 rat anti mouse cd11b - by Bioz Stars, 2026-03
    99/100 stars

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    1) Product Images from "Lupus serum IgG induces microglia activation through Fc fragment dependent way and modulated by B-cell activating factor"

    Article Title: Lupus serum IgG induces microglia activation through Fc fragment dependent way and modulated by B-cell activating factor

    Journal: Journal of Translational Medicine

    doi: 10.1186/s12967-019-02175-0

    Flow cytometric analysis of microglia surface markers in SLE-serum, healthy-serum and ACSF-treated mice (n = 16, 14 and 10). a Cells were defined by gate R1 to eliminate unwanted events, such as cell debris. b Microglias were identified by their CD45 low CD11b + phenotype (R2). c Isotype for MHCII was used to define the gating of M1 cells. In this population, SLE-serum led to a significantly increased percentage of MHC-II + CD45 low CD11b + cells. d Isotype for CD206 was used to define the gating of M2 cells. In contrast, percentage of CD206 + was not significantly changed by SLE-serum treatment in the CD45 low CD11b + cells. Bars represent the mean ± SEM of mice groups received injection of SLE-serum, healthy-serum or ACSF. Pictures of c and d show representative histogram and flow dot plots of MHC-II and CD206 expression, respectively, in the CD45 low CD11b + population. *p < 0.05; **p < 0.01
    Figure Legend Snippet: Flow cytometric analysis of microglia surface markers in SLE-serum, healthy-serum and ACSF-treated mice (n = 16, 14 and 10). a Cells were defined by gate R1 to eliminate unwanted events, such as cell debris. b Microglias were identified by their CD45 low CD11b + phenotype (R2). c Isotype for MHCII was used to define the gating of M1 cells. In this population, SLE-serum led to a significantly increased percentage of MHC-II + CD45 low CD11b + cells. d Isotype for CD206 was used to define the gating of M2 cells. In contrast, percentage of CD206 + was not significantly changed by SLE-serum treatment in the CD45 low CD11b + cells. Bars represent the mean ± SEM of mice groups received injection of SLE-serum, healthy-serum or ACSF. Pictures of c and d show representative histogram and flow dot plots of MHC-II and CD206 expression, respectively, in the CD45 low CD11b + population. *p < 0.05; **p < 0.01

    Techniques Used: Injection, Expressing



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    Image Search Results


    Flow cytometric analysis of microglia surface markers in SLE-serum, healthy-serum and ACSF-treated mice (n = 16, 14 and 10). a Cells were defined by gate R1 to eliminate unwanted events, such as cell debris. b Microglias were identified by their CD45 low CD11b + phenotype (R2). c Isotype for MHCII was used to define the gating of M1 cells. In this population, SLE-serum led to a significantly increased percentage of MHC-II + CD45 low CD11b + cells. d Isotype for CD206 was used to define the gating of M2 cells. In contrast, percentage of CD206 + was not significantly changed by SLE-serum treatment in the CD45 low CD11b + cells. Bars represent the mean ± SEM of mice groups received injection of SLE-serum, healthy-serum or ACSF. Pictures of c and d show representative histogram and flow dot plots of MHC-II and CD206 expression, respectively, in the CD45 low CD11b + population. *p < 0.05; **p < 0.01

    Journal: Journal of Translational Medicine

    Article Title: Lupus serum IgG induces microglia activation through Fc fragment dependent way and modulated by B-cell activating factor

    doi: 10.1186/s12967-019-02175-0

    Figure Lengend Snippet: Flow cytometric analysis of microglia surface markers in SLE-serum, healthy-serum and ACSF-treated mice (n = 16, 14 and 10). a Cells were defined by gate R1 to eliminate unwanted events, such as cell debris. b Microglias were identified by their CD45 low CD11b + phenotype (R2). c Isotype for MHCII was used to define the gating of M1 cells. In this population, SLE-serum led to a significantly increased percentage of MHC-II + CD45 low CD11b + cells. d Isotype for CD206 was used to define the gating of M2 cells. In contrast, percentage of CD206 + was not significantly changed by SLE-serum treatment in the CD45 low CD11b + cells. Bars represent the mean ± SEM of mice groups received injection of SLE-serum, healthy-serum or ACSF. Pictures of c and d show representative histogram and flow dot plots of MHC-II and CD206 expression, respectively, in the CD45 low CD11b + population. *p < 0.05; **p < 0.01

    Article Snippet: Then, the following antibodies were used for mouse microglia surface staining: PE-Cy7 rat anti-mouse CD45 (#130-110-799, MiltenyiBiotec, BergischGladbach, Germany), APC-Cy7 rat anti-mouse CD11b (#130-109-366, MiltenyiBiotec, BergischGladbach, Germany), FITC rat anti-mouse MHCII (#11-5322-81, Invirogen, Carlsbad, USA), isotype for MHCII (#11-4031-81, Invirogen, Carlsbad, USA), Percp-cy5.5 rat anti-mouse CD206 (#141715, BioLegend, San Diego, USA) and isotype for CD206 (#400531, BioLegend, San Diego, USA).

    Techniques: Injection, Expressing

    Representative fluorescent images of F4/80 marker (green, left panel). Representative fluorescent images of CD11b marker (green, right panel). Scale bar = 100 μm.

    Journal: PLoS ONE

    Article Title: Characterization of hepatic macrophages and evaluation of inflammatory response in heme oxygenase-1 deficient mice exposed to scAAV9 vectors

    doi: 10.1371/journal.pone.0240691

    Figure Lengend Snippet: Representative fluorescent images of F4/80 marker (green, left panel). Representative fluorescent images of CD11b marker (green, right panel). Scale bar = 100 μm.

    Article Snippet: Then, the cells were incubated for 30 minutes at 4°C with the following antibody mix: BV605-conjugated rat anti-mouse CD45 (clone: 30-F11; BD Biosciences), AF700-conjugated rat anti-mouse CD11c (Clone: N418, BD Biosciences); APC-Cy7-conjugated rat anti-mouse CD11b (Clone: M1/70, BD Biosciences) and APC-conjugated rat anti-mouse F4/80 (Clone: BM8, BD Biosciences).

    Techniques: Marker

    (A) Gating strategy and representative dot plots demonstrating F4/80 low CD11b high (MDMs) and F4/80 high CD11b low (KCs). Flow cytometric analysis of KCs (B) and MDMs (C) 3 days after first and 3 days after second administration of scAAV9 vectors. Values represent means ± SEM (n = 3–4 mice/group). *p < 0.05 vs. appropriate WT; † p < 0.05 vs. appropriate untreated; # p < 0.05 vs. AAV9-GFP after the 1 st administration.

    Journal: PLoS ONE

    Article Title: Characterization of hepatic macrophages and evaluation of inflammatory response in heme oxygenase-1 deficient mice exposed to scAAV9 vectors

    doi: 10.1371/journal.pone.0240691

    Figure Lengend Snippet: (A) Gating strategy and representative dot plots demonstrating F4/80 low CD11b high (MDMs) and F4/80 high CD11b low (KCs). Flow cytometric analysis of KCs (B) and MDMs (C) 3 days after first and 3 days after second administration of scAAV9 vectors. Values represent means ± SEM (n = 3–4 mice/group). *p < 0.05 vs. appropriate WT; † p < 0.05 vs. appropriate untreated; # p < 0.05 vs. AAV9-GFP after the 1 st administration.

    Article Snippet: Then, the cells were incubated for 30 minutes at 4°C with the following antibody mix: BV605-conjugated rat anti-mouse CD45 (clone: 30-F11; BD Biosciences), AF700-conjugated rat anti-mouse CD11c (Clone: N418, BD Biosciences); APC-Cy7-conjugated rat anti-mouse CD11b (Clone: M1/70, BD Biosciences) and APC-conjugated rat anti-mouse F4/80 (Clone: BM8, BD Biosciences).

    Techniques:

    KEY RESOURCES TABLE

    Journal: Immunity

    Article Title: microRNA-142 is critical for the homeostasis and function of type-1 innate lymphoid cells

    doi: 10.1016/j.immuni.2019.06.016

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: APC CY7 Rat anti-mouse CD11b mAb (M1/70) , BD Biosciences , Cat# 557657.

    Techniques: Recombinant, Derivative Assay, Electron Microscopy, Cell Isolation, Flow Cytometry, Luciferase, Microarray, Control Assay, Software

    Antibody fluorophore and clones (see step 4.9). Generally, one sample is either one or two digested eyes. Add flow buffer to a tube first then each antibody. Dilutions can be made to avoid pipetting too small a volume, while changing total flow buffer volume appropriately. See for product number and company.

    Journal: Journal of visualized experiments : JoVE

    Article Title: Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes

    doi: 10.3791/61348

    Figure Lengend Snippet: Antibody fluorophore and clones (see step 4.9). Generally, one sample is either one or two digested eyes. Add flow buffer to a tube first then each antibody. Dilutions can be made to avoid pipetting too small a volume, while changing total flow buffer volume appropriately. See for product number and company.

    Article Snippet: Rat anti-mouse CD11b APC-Cy7 , BD Biosciences , 557657 , .

    Techniques: Clone Assay

    Journal: Journal of visualized experiments : JoVE

    Article Title: Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes

    doi: 10.3791/61348

    Figure Lengend Snippet:

    Article Snippet: Rat anti-mouse CD11b APC-Cy7 , BD Biosciences , 557657 , .

    Techniques: Electron Microscopy, Software, Microscopy, Blocking Assay, Flow Cytometry

    Configuration of flow cytometer. Filter and mirror setup for a four-laser cytometer and the available fluorophores which can be detected in each channel. PMT = photomultiplier tube, FSC = forward scatter, SSC = side scatter.

    Journal: Journal of visualized experiments : JoVE

    Article Title: Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes

    doi: 10.3791/61348

    Figure Lengend Snippet: Configuration of flow cytometer. Filter and mirror setup for a four-laser cytometer and the available fluorophores which can be detected in each channel. PMT = photomultiplier tube, FSC = forward scatter, SSC = side scatter.

    Article Snippet: Rat anti-mouse CD11b APC-Cy7 , BD Biosciences , 557657 , .

    Techniques: Flow Cytometry, Cytometry

    Antibody fluorophore and clones for single color controls. Each antibody should be added to the appropriate compensation bead as outlined in steps 5.3.1 – 5.3.3.

    Journal: Journal of visualized experiments : JoVE

    Article Title: Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes

    doi: 10.3791/61348

    Figure Lengend Snippet: Antibody fluorophore and clones for single color controls. Each antibody should be added to the appropriate compensation bead as outlined in steps 5.3.1 – 5.3.3.

    Article Snippet: Rat anti-mouse CD11b APC-Cy7 , BD Biosciences , 557657 , .

    Techniques: Clone Assay

    (A) Single color controls (SCCs) for Alexa647. On the left, the SCC for Alexa647 is shown. Left middle displays the spill of the Alexa647 SCC into the Alexa700 channel. The peak of the Alexa647 SCC is shown in magenta and the 0.5 Log goal differential is displayed in cyan. Note that the peak is to the left (less bright) than the cyan line. Middle right demonstrates the spill of the Alexa647 SCC into the APC-Cy7 channel. Right illustrates the cells in the Alexa647 channel. Note that all cells are less bright than the SCC (magenta line). (B) SCC for Alexa700. Middle left shows the SCC for Alexa700. Left displays the spill of the Alexa700 SCC into the Alexa647 channel. Middle right demonstrates the spill of the Alexa700 SCC into the APC-Cy7 channel. The peak of the Alexa700 SCC is shown in magenta and the 0.5 Log goal differential is displayed in cyan. Note that the peak is to the left (less bright) than the cyan line. Right illustrates the cells in the Alexa700 channel. Note that all cells are less bright than the SCC (magenta line). (C) SCC for APC-Cy7. Left and middle left show the spill of the APC-Cy7 SCC into Alexa647 and Alexa700, respectively. Note that both peaks are to the left of the cyan line. Middle right demonstrates the ACP-Cy7 SCC. Right illustrates the cells in the APC-Cy7 channel. Note that all cells are less bright than the SCC (magenta line).

    Journal: Journal of visualized experiments : JoVE

    Article Title: Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes

    doi: 10.3791/61348

    Figure Lengend Snippet: (A) Single color controls (SCCs) for Alexa647. On the left, the SCC for Alexa647 is shown. Left middle displays the spill of the Alexa647 SCC into the Alexa700 channel. The peak of the Alexa647 SCC is shown in magenta and the 0.5 Log goal differential is displayed in cyan. Note that the peak is to the left (less bright) than the cyan line. Middle right demonstrates the spill of the Alexa647 SCC into the APC-Cy7 channel. Right illustrates the cells in the Alexa647 channel. Note that all cells are less bright than the SCC (magenta line). (B) SCC for Alexa700. Middle left shows the SCC for Alexa700. Left displays the spill of the Alexa700 SCC into the Alexa647 channel. Middle right demonstrates the spill of the Alexa700 SCC into the APC-Cy7 channel. The peak of the Alexa700 SCC is shown in magenta and the 0.5 Log goal differential is displayed in cyan. Note that the peak is to the left (less bright) than the cyan line. Right illustrates the cells in the Alexa700 channel. Note that all cells are less bright than the SCC (magenta line). (C) SCC for APC-Cy7. Left and middle left show the spill of the APC-Cy7 SCC into Alexa647 and Alexa700, respectively. Note that both peaks are to the left of the cyan line. Middle right demonstrates the ACP-Cy7 SCC. Right illustrates the cells in the APC-Cy7 channel. Note that all cells are less bright than the SCC (magenta line).

    Article Snippet: Rat anti-mouse CD11b APC-Cy7 , BD Biosciences , 557657 , .

    Techniques:

    (A) Side scatter area (SSC-A) vs forward scatter area (FSC-A) for all cells on a contour plot. Count beads are identified by high SSC-A, low FSC-A, and very tight clustering of the uniform beads. (B) PE vs APC-Cy7 plot for clean bead gate. The arrow between A and B indicates plotting of only the count bead positive events. Clean count beads are delineated by high PE and low APC-Cy7 fluorescence. (C) FSC-Height (FSC-H) vs FSC-Area (FSC-A) plot of all cells demonstrates singlet cells in a positively correlated wide line. Doublets and other multiplets show greater FSC-Area than FSC-Height. (D) Live / Dead vs FSC-A for singlet cells. Live cells are defined as Live / Dead low and FSC-A positive. Percentages indicate percent of parent.

    Journal: Journal of visualized experiments : JoVE

    Article Title: Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes

    doi: 10.3791/61348

    Figure Lengend Snippet: (A) Side scatter area (SSC-A) vs forward scatter area (FSC-A) for all cells on a contour plot. Count beads are identified by high SSC-A, low FSC-A, and very tight clustering of the uniform beads. (B) PE vs APC-Cy7 plot for clean bead gate. The arrow between A and B indicates plotting of only the count bead positive events. Clean count beads are delineated by high PE and low APC-Cy7 fluorescence. (C) FSC-Height (FSC-H) vs FSC-Area (FSC-A) plot of all cells demonstrates singlet cells in a positively correlated wide line. Doublets and other multiplets show greater FSC-Area than FSC-Height. (D) Live / Dead vs FSC-A for singlet cells. Live cells are defined as Live / Dead low and FSC-A positive. Percentages indicate percent of parent.

    Article Snippet: Rat anti-mouse CD11b APC-Cy7 , BD Biosciences , 557657 , .

    Techniques: Fluorescence

    Left: CD45 vs CD11b pseudocolor plot of live cells from unlasered (A), lasered (B), and fluorescence minus one (FMO) control (C). Left: CD45+ cells are defined in A-B and absent in the FMO. Note the increase of CD45+ CD11b+ cells in the lasered (B) group. Middle: Pseudocolor plot of lineage (Lin) marker vs CD11b for CD45+ cells. CD11b+ Lin− cells are delineated in A-B and are absent in the FMO for CD11b (bottom). Right: CD11b vs CD45 plot of CD11b+ Lin− cells. CD45dim and CD45high cells are identified. Note the increase in CD45high cells in the lasered (B) group. Percentages indicate percent of parent.

    Journal: Journal of visualized experiments : JoVE

    Article Title: Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes

    doi: 10.3791/61348

    Figure Lengend Snippet: Left: CD45 vs CD11b pseudocolor plot of live cells from unlasered (A), lasered (B), and fluorescence minus one (FMO) control (C). Left: CD45+ cells are defined in A-B and absent in the FMO. Note the increase of CD45+ CD11b+ cells in the lasered (B) group. Middle: Pseudocolor plot of lineage (Lin) marker vs CD11b for CD45+ cells. CD11b+ Lin− cells are delineated in A-B and are absent in the FMO for CD11b (bottom). Right: CD11b vs CD45 plot of CD11b+ Lin− cells. CD45dim and CD45high cells are identified. Note the increase in CD45high cells in the lasered (B) group. Percentages indicate percent of parent.

    Article Snippet: Rat anti-mouse CD11b APC-Cy7 , BD Biosciences , 557657 , .

    Techniques: Fluorescence, Marker